E1218-97a* Standard Guide for Conducting Static 96-h Toxicity Tests with Microalgae
Copyright 1997 AMERICAN SOCIETY FOR TESTING AND MATERIALS, West Conshohocken, PA. All rights reserved.
1.1 This guide describes procedures for obtaining laboratory data concerning the adverse effects of a test material added to dilution water on growth of certain species of freshwater and saltwater microalgae during a 96-h static exposure. These procedures will probably be useful for conducting short-term toxicity tests with other species of algae, although modifications might be necessary.
1.2 Other modifications of these procedures might be justified by special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, results of tests conducted using unusual procedures are not likely to be comparable to results of many other tests. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting toxicity tests with microalgae.
1.3 These procedures are applicable to many chemicals, either individually or in formulations, commercial products, or known mixtures. With appropriate modifications, these procedures can be used to conduct tests on temperature, dissolved oxygen, and pH and on such materials as aqueous effluents (see also Guide E1192), leachates, oils, particulate matter, sediments, and surface waters. Static tests might not be applicable to materials that have a high oxygen demand, are highly volatile, are rapidly biologically or chemically transformed in aqueous solutions, or are removed from test solutions in substantial quantities by the test chambers or organisms during the test.
1.4 Results of tests using microalgae should usually be reported in terms of the 96-h IC50 (see 3.1.4) based on reduction in growth. In some situations, it might only be necessary to determine whether a specific concentration unacceptably affects the growth of the test species or whether the IC50 is above or below a specific concentration.
1.5 This guide is arranged as follows:
Section Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Hazards 6 Apparatus 7 Facilities 7.1 Equipment 7.2 Test Chambers 7.3 Cleaning 7.4 Acceptability 7.5 Dilution Water 8 Materials 9 General 9.1 Stock Solutions 9.2 Test Concentration(s) 9.3 Test Organisms 10 Species 10.1 Source 10.2 Culture 10.3 Quality 10.4 Procedure 11 Experimental Design 11.1 Temperature 11.2 Illumination 11.3 Beginning the Test 11.4 Gas Exchange 11.5 Duration of Test 11.6 Biological Data 11.7 Other Measurements 11.8 Analytical Methodology 12 Acceptability of Test 13 Calculation 14 Report 15
1.6 This standard does not purport to address all of the safety problems associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific hazard statements are given in Section 6.
*Appears in - Day, et al Review of whole-organism bioassays: Soil, freshwater sediment, and freshwater assessment in Canada. Ecotoxicology and Environmental Safety, v.30, n.3, (1995): 221-251.